產(chǎn)品時間2024-11-14
瀏覽量91
所屬分類紫外可見分光光度計
報價68888
品牌 | 析譜 | 光譜帶寬 | 1.8nm |
---|---|---|---|
波長準確度 | 0.5nm | 雜散光(S.L.) | 0.02%T |
波長范圍 | 190-1100nm | 自動程度 | 自動波長 |
接收器類 | 光電倍增管(PMT) | 儀器結構 | 雙波長 |
價格區(qū)間 | 5千-1萬 | 應用領域 | 環(huán)保,生物產(chǎn)業(yè),石油,能源,制藥 |
冷卻液氯離子自動檢測儀紫外可見分光光度計介紹:
所輸入的因子個數(shù)與所選擇的曲線擬合方法有關,下表是其對應關系:
曲線擬合方法 | 曲線方程表達式 | 所需輸入的因子數(shù) |
一階線性過零擬合 | C=K1×A | K1, r* |
一階線性擬合 | C=K0+K1×A | K0,K1,r* |
二階擬合 | C=K0+K1×A+K2×A2 | K0,K1,K2 |
三階擬合 | C=K0+K1×A+K2×A2+K3×A3 | K0,K1,K2,K3 |
試驗前的準備
? 將試驗用比色皿或試管用蒸餾水或其他專門的清洗劑清洗干凈,并用柔軟的棉布或紙巾將其表面的手指印或滴液擦試干凈;
? 將盛參比液的比色皿放入4聯(lián)手動樣品架(紫外可見分光光度計系列)或固定單個樣品架(對于雙光束紫外可見分光光度計)最靠近你的槽位中,再將推桿向前推到頭使比色皿正對光路,關上樣品室蓋;
Appendix B
Correction Methods
A number of correction techniques can be used to eliminate or reduce interference errors.In general,if the source of the error is known and is consistent from sample to sample.the error can be eliminated.On the other hand,if the source is unkown and varies from sample to sample,the error can be reduced but not eliminated.Correction techniques can always require data from at least two wavelengths. The more sophisticated correction techniques require multiwavelenghth or spectral data.
A.1 Isoabsorbance
When a known interfering component with a known spectrum is present,the error introduced by this component at the analytical wavelength for the target analyte can be eliminated by selecting a reference wavelength at which the interfering compound exhibits the same absorbance as it does at the analytical wavelength.The absorbance at this reference wavelength is subtracted from the absorbance at the analytical wavelength,as shown in Figure A1.The residual absorbance is the ture absorbance of the analyte.
This technique is less reliable when the spectra of the analyte and of the interferent are highly similar.Moreover,it can correct for only one interference
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